The Compound Microscope

Life Science - Middle School

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The Compound Microscope © Copyright NewPath Learning. All Rights Reserved. 94-4706 www.newpathlearning.com Using the microscope 1. Rotate the low-power objective into its place. 2. Turn on your lamp microscope or position the mirror so that the opening in the stage is evenly illuminated. 3. Place a prepared slide on the stage and clamp it down with the stage clips. 4. Use the coarse adjustment knob to lower the stage to the lowest level. 5. While looking through the eyepiece, turn the coarse adjustment knob until the specimen on the slide is in view. Then, turn the fine adjustment to sharpen the focus. 6. Rotate the high-power objective into its place for a closer look. 7. To determine the magnification of the specimen on the slide, multiply the number inscribed on the eyepiece by the number on the objective being used. stage clip stage diaphragm base substage lamp fine adjustment coarse adjustment high-power objective revolving nosepiece low-power objective eyepiece arm Images seen under the microscope Hydra spiral bacteria bacteria mitotic cell cork lung tissue A microscope allows observation of objects that are not visible to the human eye. A light compound microscope uses two convex lenses to magnify an object. Light first passes through a specimen and then through the objective lens and the eyepiece lens. These lenses magnify an image by bending the light that passes through them. For example, eyepiece (10X) x objective (40X) = total magnification of 400X. Some photos courtesy of CDC.
The Compound Microscope © Copyright NewPath Learning. All Rights Reserved. 94-4706 www.newpathlearning.com Images seen under the microscope Hydra spiral bacteria bacteria mitotic cell cork lung tissue A ___________________ allows observation of objects that are not visible to the human eye. A light compound microscope uses two __________________________________ to ___________________ an object. ____________________ first passes through a specimen and then through the objective lens and the eyepiece lens. These lenses ___________________________ an image by ____________________ the light that passes through them. Key Vocabulary Terms arm low-power objective compound light microscope magnification convex lens microscope course adjustment objective diaphragm revolving nosepiece eyepiece stage fine adjustment stage clip high-power objective substage lamp Using the microscope 1. 2. 3. 4. 5. 6. 7. ______________________________________ ______________________________________ ______________________________________ ______________________________________ ______________________________________ ______________________________________ ______________________________________ ______________________________________ ______________________________________ ______________________________________ ______________________________________ ______________________________________ ______________________________________ ______________________________________ ______________________________________ ______________________________________ ______________________________________ ______________________________________ \|xiBAHBDy01774lz[